MHC Class II Polyclonal Antibody, AbBy Fluor-647 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-8481R-BF647-100uL |
| Product Name | MHC Class II Polyclonal Antibody, AbBy Fluor-647 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat |
| Specifications | |
| Conjugation | AbBy Fluor-647 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human HLA class II histocompatibility antigen DM beta chain |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3109 |
| Swiss Prot | P28068 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | RING7; D6S221E; HLA class II histocompatibility antigen, DM beta chain; MHC class II antigen DMB; Really interesting new gene 7 protein; HLA-DMB; DMB |
| Background | Plays a critical role in catalyzing the release of class II-associated invariant chain peptide (CLIP) from newly synthesized MHC class II molecules and freeing the peptide binding site for acquisition of antigenic peptides. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is MHC Class II Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-8481R-BF647) validated for, and at what dilution?
bs-8481R-BF647 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does MHC Class II Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-8481R-BF647) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat.
How should bs-8481R-BF647 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise MHC Class II Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-8481R-BF647)?
KLH conjugated synthetic peptide derived from human HLA class II histocompatibility antigen DM beta chain
What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bs-8481R-BF647)?
AbBy Fluor-647 on bs-8481R-BF647: excitation / emission 650nm/665nm.