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FNDC5 Polyclonal Antibody, PE Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse

Predicted Reactivity

  • Rat
  • Dog
  • Chicken
  • Rabbit
Overview
Catalog # bs-8486R-PE
Product Name FNDC5 Polyclonal Antibody, PE Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Mouse
Predicted Reactivity Rat, Dog, Chicken, Rabbit
Specifications
Conjugation PE
Host Rabbit
Source KLH conjugated synthetic peptide derived from human FNDC5 Irisin
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 252995
Swiss Prot Q8NAU1
Subcellular location Cytoplasm, Secreted, Cell membrane
Synonyms FRCP2; irisin; Fibronectin type III domain-containing protein 5; Fibronectin type III repeat-containing protein 2; FNDC5
Background Irisin: Contrary to mouse, may not be involved in the beneficial effects of muscular exercise, nor in the induction of browning of human white adipose tissue.
Application Dilution
WB 1:300-5000
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Frequently asked questions

Which applications is FNDC5 Polyclonal Antibody, PE Conjugated (bs-8486R-PE) validated for, and at what dilution?

bs-8486R-PE is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does FNDC5 Polyclonal Antibody, PE Conjugated (bs-8486R-PE) react with?

Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Chicken, Rabbit.

How should bs-8486R-PE be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise FNDC5 Polyclonal Antibody, PE Conjugated (bs-8486R-PE)?

KLH conjugated synthetic peptide derived from human FNDC5 Irisin

What are the excitation and emission wavelengths of the PE conjugate (bs-8486R-PE)?

PE on bs-8486R-PE: excitation / emission 496,564nm/578nm.