FBXO25 Polyclonal Antibody, Biotin Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-8491R-Biotin-100uL |
| Product Name | FBXO25 Polyclonal Antibody, Biotin Conjugated |
| Applications |
WB
ELISA
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Pig, Rabbit |
| Specifications | |
| Conjugation | Biotin |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human FBXO25 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C for 12 months. |
| Target | |
| Gene ID | 26260 |
| Subcellular location | Nucleus |
| Synonyms | F box only protein 25; F box protein 25; F box protein Fbx25; F-box only protein 25; FBX25; MGC20256; MGC51975; OTTHUMP00000115399; |
| Background | FBXO25 is a member of the F box protein family which is characterized by an approximately 40 amino acid motif, the F box. The F box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1 cullin F box), which function in phosphorylation dependent ubiquitination. The F box proteins are divided into 3 classes: Fbws containing WD 40 domains, Fbls containing leucine rich repeats, and Fbxs containing either different protein protein interaction modules or no recognizable motifs. FBXO25 belongs to the Fbxs class. There are three named isoforms produced by alternative splicing.FBXO25, also known as FBX25, is a 367 amino acid protein that contains one C-terminal F-box domain and belongs to the Fbx class of the F-box family of proteins. F-box proteins are critical components of the SCF (Skp1-CUL-1-F-box protein) type E3 ubiquitin ligase complex and are involved in substrate recognition and recruitment for ubiquitination. They are members of a larger family of proteins that are involved in the regulation of a wide variety of cellular processes (including the cell cycle, immune response, signaling cascades and developmental processes) through the targeting of proteins, such as cyclins, cyclin-dependent kinase inhibitors, IkB-a and b-catenin, for degradation by the proteasome after ubiquitination. Expressed at high levels in brain, FBXO25 localizes predominantly to the nucleus and directly interacts with Skp1 p19 and CUL-1. Disruption of the gene encoding FBXO25 can lead to X-linked mental retardation. |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
Frequently asked questions
Which applications is FBXO25 Polyclonal Antibody, Biotin Conjugated (bs-8491R-Biotin) validated for, and at what dilution?
bs-8491R-Biotin is validated for WB (1:300-5000); ELISA (1:500-1000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does FBXO25 Polyclonal Antibody, Biotin Conjugated (bs-8491R-Biotin) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Pig, Rabbit.
How should bs-8491R-Biotin be stored?
Store at -20C for 12 months. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise FBXO25 Polyclonal Antibody, Biotin Conjugated (bs-8491R-Biotin)?
KLH conjugated synthetic peptide derived from human FBXO25