XRCC4 Polyclonal Antibody, PE-Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-8510R-PE-Cy5.5-100uL |
| Product Name | XRCC4 Polyclonal Antibody, PE-Cy5.5 Conjugated |
| Applications |
WB
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Cow, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | PE-Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human XRCC4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 7518 |
| Swiss Prot | Q13426 |
| Subcellular location | Nucleus |
| Synonyms | DNA repair protein XRCC4; X-ray repair cross-complementing protein 4; XRCC4 |
| Background | Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. Binds to DNA and to DNA ligase IV (LIG4). The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is XRCC4 Polyclonal Antibody, PE-Cy5.5 Conjugated (BS-8510R-PE-CY5-5) validated for, and at what dilution?
BS-8510R-PE-CY5-5 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does XRCC4 Polyclonal Antibody, PE-Cy5.5 Conjugated (BS-8510R-PE-CY5-5) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Pig, Horse, Rabbit.
How should BS-8510R-PE-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise XRCC4 Polyclonal Antibody, PE-Cy5.5 Conjugated (BS-8510R-PE-CY5-5)?
KLH conjugated synthetic peptide derived from human XRCC4
What are the excitation and emission wavelengths of the PE-Cy5.5 conjugate (BS-8510R-PE-CY5-5)?
PE-Cy5.5 on BS-8510R-PE-CY5-5: excitation / emission 488nm/694nm.