SPP24 Polyclonal Antibody, AbBy Fluor-488 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-8518R-BF488-100uL |
| Product Name | SPP24 Polyclonal Antibody, AbBy Fluor-488 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human |
| Specifications | |
| Conjugation | AbBy Fluor-488 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human SPP24/SPP2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 6694 |
| Swiss Prot | Q13103 |
| Subcellular location | Secreted |
| Synonyms | SPP24; SPP-24; Secreted phosphoprotein 24; Secreted phosphoprotein 2; SPP2 |
| Background | SPP24, also known as secreted phosphoprotein 2, is a 211 amino acid secreted protein that belongs to the cystatin superfamily. Expressed in liver and plasma, SPP24 may play a role in coordinating an aspect of bone turnover. The gene that encodes SPP24 maps to human chromosome 2, which consists of 237 million bases encoding over 1,400 genes, making up approximately 8% of the human genome. A number of genetic diseases are linked to genes on chromosome 2. Harlequin icthyosis, a rare and morbid skin deformity, is associated with mutations in the ABCA12 gene. The lipid metabolic disorder sitosterolemia is associated with ABCG5 and ABCG8. An extremely rare recessive genetic disorder, Alstr syndrome is due to mutations in the ALMS1 gene. Interestingly, chromosome 2 contains what appears to be a vestigial second centromere and vestigial telomeres which gives credence to the hypothesis that human chromosome 2 is the result of an ancient fusion of two ancestral chromosomes seen in modern form today in apes. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is SPP24 Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-8518R-BF488) validated for, and at what dilution?
bs-8518R-BF488 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-8518R-BF488 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise SPP24 Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-8518R-BF488)?
KLH conjugated synthetic peptide derived from human SPP24/SPP2
What are the excitation and emission wavelengths of the AbBy Fluor-488 conjugate (bs-8518R-BF488)?
AbBy Fluor-488 on bs-8518R-BF488: excitation / emission 499nm/519nm.