MtTF1/TFAM Polyclonal Antibody, AbBy Fluor-488 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-8520R-BF488 |
| Product Name | MtTF1/TFAM Polyclonal Antibody, AbBy Fluor-488 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-488 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human MtTF1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 7019 |
| Swiss Prot | Q00059 |
| Subcellular location | Cytoplasm |
| Synonyms | TCF6; MTTF1; MTTFA; TCF6L1; TCF6L2; TCF6L3; Transcription factor A, mitochondrial; Mitochondrial transcription factor 1; Transcription factor 6; TCF-6; Transcription factor 6-like 2; TFAM |
| Background | Binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites. Is able to unwind DNA. Bends the mitochondrial light strand promoter DNA into a U-turn shape via its HMG boxes. Required for maintenance of normal levels of mitochondrial DNA. May play a role in organizing and compacting mitochondrial DNA. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is MtTF1/TFAM Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-8520R-BF488) validated for, and at what dilution?
bs-8520R-BF488 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does MtTF1/TFAM Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-8520R-BF488) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat.
How should bs-8520R-BF488 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise MtTF1/TFAM Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-8520R-BF488)?
KLH conjugated synthetic peptide derived from human MtTF1
What are the excitation and emission wavelengths of the AbBy Fluor-488 conjugate (bs-8520R-BF488)?
AbBy Fluor-488 on bs-8520R-BF488: excitation / emission 499nm/519nm.