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STIM1 Polyclonal Antibody, Biotin Conjugated

Applications

  • ELISA
  • IHC-P
  • IHC-F

Reactivity

  • Rat

Predicted Reactivity

  • Human
  • Mouse
  • Dog
  • Cow
  • Pig
  • Horse
  • Chicken
  • Rabbit
Overview
Catalog # bs-8526R-Biotin
Product Name STIM1 Polyclonal Antibody, Biotin Conjugated
Applications
ELISA IHC-P IHC-F
Specificity May cross react with STIM2
Reactivity Rat
Predicted Reactivity Human, Mouse, Dog, Cow, Pig, Horse, Chicken, Rabbit
Specifications
Conjugation Biotin
Host Rabbit
Source KLH conjugated synthetic peptide derived from human STIM1
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C for 12 months.
Target
Gene ID 6786
Swiss Prot Q13586
Subcellular location Cytoplasm, Cell membrane
Synonyms GOK; TAM; TAM1; IMD10; STRMK; D11S4896E; Stromal interaction molecule 1; STIM1
Background Plays a role in mediating store-operated Ca(2+) entry (SOCE), a Ca(2+) influx following depletion of intracellular Ca(2+) stores. Acts as Ca(2+) sensor in the endoplasmic reticulum via its EF-hand domain. Upon Ca(2+) depletion, translocates from the endoplasmic reticulum to the plasma membrane where it activates the Ca(2+) release-activated Ca(2+) (CRAC) channel subunit, TMEM142A/ORAI1.
Application Dilution
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500

Frequently asked questions

Which applications is STIM1 Polyclonal Antibody, Biotin Conjugated (bs-8526R-Biotin) validated for, and at what dilution?

bs-8526R-Biotin is validated for ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does STIM1 Polyclonal Antibody, Biotin Conjugated (bs-8526R-Biotin) react with?

Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Dog, Cow, Pig, Horse, Chicken, Rabbit.

How should bs-8526R-Biotin be stored?

Store at -20C for 12 months. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise STIM1 Polyclonal Antibody, Biotin Conjugated (bs-8526R-Biotin)?

KLH conjugated synthetic peptide derived from human STIM1