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SDHC Polyclonal Antibody, AbBy Fluor-680 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Predicted Reactivity

  • Human
Overview
Catalog # bs-8530R-BF680-100uL
Product Name SDHC Polyclonal Antibody, AbBy Fluor-680 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Predicted Reactivity Human
Specifications
Conjugation AbBy Fluor-680
Host Rabbit
Source KLH conjugated synthetic peptide derived from human SDHC
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 6391
Swiss Prot Q99643
Subcellular location Cytoplasm, Cell membrane
Synonyms CYBL; PGL3; QPS1; SDH3; CYB560; Succinate dehydrogenase cytochrome b560 subunit, mitochondrial; Integral membrane protein CII-3; QPs-1; Succinate dehydrogenase complex subunit C; Succinate-ubiquinone oxidoreductase cytochrome B large subunit; SDHC
Background Membrane-anchoring subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q).
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is SDHC Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-8530R-BF680) validated for, and at what dilution?

bs-8530R-BF680 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

How should bs-8530R-BF680 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise SDHC Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-8530R-BF680)?

KLH conjugated synthetic peptide derived from human SDHC

What are the excitation and emission wavelengths of the AbBy Fluor-680 conjugate (bs-8530R-BF680)?

AbBy Fluor-680 on bs-8530R-BF680: excitation / emission 679nm/702nm.

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