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Nucleolin/C23 Polyclonal Antibody, FITC Conjugated

Molt-4 cells were fixed with 4% PFA for 10min at room temperature, permeabilized with 20% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with C23 Polyclonal Antibody, FITC Conjugated (bs-8536R-FITC) at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green) and isotype control (orange).
Molt-4 cells were fixed with 4% PFA for 10min at room temperature, permeabilized with 20% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with C23 Polyclonal Antibody, FITC Conjugated (bs-8536R-FITC) at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green) and isotype control (orange).
Bioss conjugated antibody and packaging; contents and labels vary by product.
Bioss conjugated antibody and packaging; contents and labels vary by product.

Applications

  • FCM
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Rat

Predicted Reactivity

  • Mouse
  • Dog
  • Cow
  • Pig
  • Horse
Overview
Catalog # bs-8536R-FITC-100uL
Product Name Nucleolin/C23 Polyclonal Antibody, FITC Conjugated
Applications
FCM IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Rat
Predicted Reactivity Mouse, Dog, Cow, Pig, Horse
Specifications
Conjugation FITC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Nucleolin
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 4691
Swiss Prot P19338
Subcellular location Cytoplasm, Nucleus
Synonyms C23; Nucleolin; Protein C23; NCL
Background Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.
Application Dilution
FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is Nucleolin/C23 Polyclonal Antibody, FITC Conjugated (bs-8536R-FITC) validated for, and at what dilution?

bs-8536R-FITC is validated for FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Nucleolin/C23 Polyclonal Antibody, FITC Conjugated (bs-8536R-FITC) react with?

Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Dog, Cow, Pig, Horse.

How should bs-8536R-FITC be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Nucleolin/C23 Polyclonal Antibody, FITC Conjugated (bs-8536R-FITC)?

KLH conjugated synthetic peptide derived from human Nucleolin

What are the excitation and emission wavelengths of the FITC conjugate (bs-8536R-FITC)?

FITC on bs-8536R-FITC: excitation / emission 494nm/518nm.

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