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ZNF537 Polyclonal Antibody, APC-Cy7 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Predicted Reactivity

  • Human
  • Mouse
  • Rat
  • Cow
  • Pig
  • Horse
  • Chicken
  • Rabbit
Overview
Catalog # bs-8540R-APC-Cy7
Product Name ZNF537 Polyclonal Antibody, APC-Cy7 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Predicted Reactivity Human, Mouse, Rat, Cow, Pig, Horse, Chicken, Rabbit
Specifications
Conjugation APC-Cy7
Host Rabbit
Source KLH conjugated synthetic peptide derived from human TSHZ3/ZNF537
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 57616
Subcellular location Nucleus
Synonyms teashirt homolog 3; Zinc finger protein 537; TSHZ3; TSH3; ZNF537; TSH3_HUMAN.
Background The homeobox DNA-binding domain is a 60 amino acid motif that is conserved among many species and functions to bind DNA via a helix-turn-helix structure, thereby playing a role in transcriptional regulation and the control of gene expression. TSHZ3 (teashirt zinc finger homeobox 3), also known as KIAA1474, TSH3 or ZNF537, is a 1,081 amino acid protein that contains one homeobox DNA-binding domain and five C2H2-type zinc fingers. Localized to the nucleus, TSHZ3 functions as a transcriptional regulator that is involved in developmental processes throughout the body. The gene encoding TSHZ3 maps to human chromosome 19, which consists of over 63 million bases, houses approximately 1,400 genes and is recognized for having the greatest gene density of the human chromosomes.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is ZNF537 Polyclonal Antibody, APC-Cy7 Conjugated (bs-8540R-APC-Cy7) validated for, and at what dilution?

bs-8540R-APC-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

How should bs-8540R-APC-Cy7 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise ZNF537 Polyclonal Antibody, APC-Cy7 Conjugated (bs-8540R-APC-Cy7)?

KLH conjugated synthetic peptide derived from human TSHZ3/ZNF537

What are the excitation and emission wavelengths of the APC-Cy7 conjugate (bs-8540R-APC-Cy7)?

APC-Cy7 on bs-8540R-APC-Cy7: excitation / emission 650nm/780nm.