MSH3 Polyclonal Antibody, Cy5 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-8542R-Cy5 |
| Product Name | MSH3 Polyclonal Antibody, Cy5 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Rabbit |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human MSH3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4437 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | Divergent upstream protein; DNA mismatch repair protein; DNA mismatch repair protein Msh 3; DNA mismatch repair protein Msh3; DUC 1; DUC1; DUG; DUP; hMSH3; Mismatch repair protein 1; MRP 1; MRP1; MSH 3; MSH-3; MSH3_HUMAN; MutS homolog 3. |
| Background | Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS beta which binds to DNA mismatches thereby initiating DNA repair. When bound, the MutS beta heterodimer bends the DNA helix and shields approximately 20 base pairs. MutS beta recognizes large insertion-deletion loops (IDL) up to 13 nucleotides long. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is MSH3 Polyclonal Antibody, Cy5 Conjugated (bs-8542R-Cy5) validated for, and at what dilution?
bs-8542R-Cy5 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-8542R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise MSH3 Polyclonal Antibody, Cy5 Conjugated (bs-8542R-Cy5)?
KLH conjugated synthetic peptide derived from human MSH3
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-8542R-Cy5)?
Cy5 on bs-8542R-Cy5: excitation / emission 625,650nm/670nm.