GABR B3 Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-8544R-Cy5.5-100uL |
| Product Name | GABR B3 Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Specificity | Possible cross-reactivity with GABRB1 and GABRB2 |
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Cow, Pig, Horse, Chicken |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human GABR B3/GABA A Receptor beta 3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 2562 |
| Swiss Prot | P28472 |
| Subcellular location | Cell membrane |
| Synonyms | ECA5; Gamma-aminobutyric acid receptor subunit beta-3; GABA(A) receptor subunit beta-3; GABRB3 |
| Background | Component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the vertebrate brain. Functions also as histamine receptor and mediates cellular responses to histamine. Functions as receptor for diazepines and various anesthetics, such as pentobarbital; these are bound at a separate allosteric effector binding site. Functions as ligand-gated chloride channel. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is GABR B3 Polyclonal Antibody, Cy5.5 Conjugated (BS-8544R-CY5-5) validated for, and at what dilution?
BS-8544R-CY5-5 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does GABR B3 Polyclonal Antibody, Cy5.5 Conjugated (BS-8544R-CY5-5) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Cow, Pig, Horse, Chicken.
How should BS-8544R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise GABR B3 Polyclonal Antibody, Cy5.5 Conjugated (BS-8544R-CY5-5)?
KLH conjugated synthetic peptide derived from human GABR B3/GABA A Receptor beta 3
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-8544R-CY5-5)?
Cy5.5 on BS-8544R-CY5-5: excitation / emission 675nm/694nm.