Hemoglobin Beta Polyclonal Antibody, APC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-8554R-APC-100uL |
| Product Name | Hemoglobin Beta Polyclonal Antibody, APC Conjugated |
| Applications |
WB
|
| Specificity | Antibody has equal affinity for Human Hemoglobin Beta and Delta |
| Reactivity | Human |
| Specifications | |
| Conjugation | APC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Hemoglobin beta |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3043; 3045 |
| Swiss Prot | P68871; P02042 |
| Subcellular location | Cytoplasm, Secreted |
| Synonyms | CD113t-C; beta-globin; Hemoglobin subunit beta; Hemoglobin beta chain; HBB; Hemoglobin subunit delta; HBD; Delta-globin; Hemoglobin delta chain |
| Background | The delta (HBD) and beta (HBB) genes are normally expressed in the adult: two alpha chains plus two beta chains constitute HbA, which in normal adult life comprises about 97% of the total hemoglobin. Two alpha chains plus two delta chains constitute HbA-2, which with HbF comprises the remaining 3% of adult hemoglobin. Five beta-like globin genes are found within a 45 kb cluster on chromosome 11 in the following order: 5'-epsilon--Ggamma--Agamma--delta--beta-3'. Mutations in the delta-globin gene are associated with beta-thalassemia. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is Hemoglobin Beta Polyclonal Antibody, APC Conjugated (bs-8554R-APC) validated for, and at what dilution?
bs-8554R-APC is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Hemoglobin Beta Polyclonal Antibody, APC Conjugated (bs-8554R-APC) react with?
Tested reactivity: Human.
How should bs-8554R-APC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Hemoglobin Beta Polyclonal Antibody, APC Conjugated (bs-8554R-APC)?
KLH conjugated synthetic peptide derived from human Hemoglobin beta
What are the excitation and emission wavelengths of the APC conjugate (bs-8554R-APC)?
APC on bs-8554R-APC: excitation / emission 650nm/660nm.