ELL3 Polyclonal Antibody, AbBy Fluor-350 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-8560R-BF350-100uL |
| Product Name | ELL3 Polyclonal Antibody, AbBy Fluor-350 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Sheep, Pig, Chicken, Rabbit |
| Specifications | |
| Conjugation | AbBy Fluor-350 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ELL3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 80237 |
| Subcellular location | Nucleus |
| Synonyms | B cell translocation gene 2; BTG family member 2; Btg2; BTG2_HUMAN; Nerve growth factor inducible anti prolerative; NGF inducible anti prolerative protein PC3; NGF-inducible anti-prolerative protein PC3; PC3; Pheochromacytoma cell 3; Protein BTG2; TIS21. |
| Background | Eukaryotic RNA polymerase II mediates the synthesis of mature and functional messenger RNA. This is a multistep process, called the transcription cycle, that includes five stages: preinitiation, promoter, clearance, elongation and termination. Elongation is thought to be a critical stage for the regulation of gene expression. ELL (11-19 lysine-rich leukemia protein), also designated MEN, functions as an RNA polymerase II elongation factor that increases the rate of transcription by suppressing transient pausing by RNA polymerase II. It is also thought to regulate cellular proliferation. ELL is abundantly expressed in peripheral blood leukocytes, skeletal muscle, placenta and testis, with lower expression in spleen, thymus, heart, brain, lung, kidney, liver and ovary. ELL3 is a 397 amino acid nuclear protein that functions as an RNA polymerase II elongation factor that increases the rate of transcription by suppressing transient pausing by RNA polymerase II. Though similar to ELL and ELL2, ELL3 is exclusively expressed in testis. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is ELL3 Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-8560R-BF350) validated for, and at what dilution?
bs-8560R-BF350 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-8560R-BF350 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ELL3 Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-8560R-BF350)?
KLH conjugated synthetic peptide derived from human ELL3
What are the excitation and emission wavelengths of the AbBy Fluor-350 conjugate (bs-8560R-BF350)?
AbBy Fluor-350 on bs-8560R-BF350: excitation / emission 346nm/442nm.