GPKOW Polyclonal Antibody, AbBy Fluor-488 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-8577R-BF488-100uL |
| Product Name | GPKOW Polyclonal Antibody, AbBy Fluor-488 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | AbBy Fluor-488 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human GPKOW |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 27238 |
| Subcellular location | Nucleus |
| Synonyms | G patch domain and KOW mots; G patch domain and KOW mots containing protein; G patch domain and KOW mots-containing protein; G patch domain containing 5; G patch domain containing protein 5; G patch domain-containing protein 5; GPATC5; GPATCH5; gpkow; GPKOW_HUMAN; Protein MOS2 homolog; Protein T54; SPP2; T54 antibody T54 protein. |
| Background | GPKOW is a potential RNA-binding protein consisting of one central G patch domain and two C-terminal KOW domains. T54 is a 476 amino acid protein belonging to the MOS2 family. It is a mammalian homolog of the Arabidopsis thaliana MOS2 (modifier of SNC1, 2) nuclear protein that is required for innate immunity. Similar to A. thaliana MOS2, T54 localizes to the nucleus and contains G patch and KOW domains, suggesting that T54 may play a similar role in mammalian innate immunity. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is GPKOW Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-8577R-BF488) validated for, and at what dilution?
bs-8577R-BF488 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does GPKOW Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-8577R-BF488) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Rabbit.
How should bs-8577R-BF488 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise GPKOW Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-8577R-BF488)?
KLH conjugated synthetic peptide derived from human GPKOW
What are the excitation and emission wavelengths of the AbBy Fluor-488 conjugate (bs-8577R-BF488)?
AbBy Fluor-488 on bs-8577R-BF488: excitation / emission 499nm/519nm.