GIPC-2 Polyclonal Antibody, FITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-8586R-FITC-100uL |
| Product Name | GIPC-2 Polyclonal Antibody, FITC Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse, Dog, Cow, Sheep, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | FITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human GIPC2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Cytoplasm |
| Synonyms | GIPC 2; GIPC-2 GIPC PDZ domain containing family, member 2; GIPC2; GIPC2_HUMAN; Likely ortholog of mouse semaF cytoplasmic domain associated protein 2; PDZ domain protein GIPC2; PDZ domain-containing protein GIPC2; SemaF cytoplasmic domain associated protein 2; Semaphorin cytoplasmic domain associated protein 2; SEMCAP 2; SEMCAP2. |
| Background | GIPC2 is a 315 amino acid protein that localizes to the cytoplasm and contains one PDZ domain. Expressed at high levels in kidney and colon and at lower levels in adult liver, GIPC2 interacts with SEMA5A and is thought to function as a scaffold protein, possibly modulating cell adhesion and growth factor signaling and playing a role in tumorigenesis. The gene encoding GIPC2 maps to human chromosome 1, which spans 260 million base pairs, contains over 3,000 genes and comprises nearly 8% of the human genome. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is GIPC-2 Polyclonal Antibody, FITC Conjugated (bs-8586R-FITC) validated for, and at what dilution?
bs-8586R-FITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does GIPC-2 Polyclonal Antibody, FITC Conjugated (bs-8586R-FITC) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Dog, Cow, Sheep, Pig, Horse, Rabbit.
How should bs-8586R-FITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise GIPC-2 Polyclonal Antibody, FITC Conjugated (bs-8586R-FITC)?
KLH conjugated synthetic peptide derived from human GIPC2
What are the excitation and emission wavelengths of the FITC conjugate (bs-8586R-FITC)?
FITC on bs-8586R-FITC: excitation / emission 494nm/518nm.