C9orf72 Polyclonal Antibody, PerCP-Cy5.5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-8595R-PerCP-Cy5.5-100uL |
| Product Name | C9orf72 Polyclonal Antibody, PerCP-Cy5.5 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | PerCP-Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human C9orf72 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 203228 |
| Swiss Prot | Q96LT7 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | ALSFTD; FTDALS; Protein C9orf72; C9orf72 |
| Background | Chromosome 9 consists of about 145 million bases and 4% of the human genome and encodes nearly 900 genes. Considered to play a role in gender determination, deletion of the distal portion of 9p can lead to development of male to female sex reversal, the phenotype of a female with a male X,Y genotype. Hereditary hemorrhagic telangiectasia, which is characterized by harmful vascular defects, is associated with the chromosome 9 gene encoding endoglin protein, ENG. Familial dysautonomia is also associated with chromosome 9 though through the gene IKBKAP. Notably, chromosome 9 encompasses the largest interferon family gene cluster. Chromosome 9 is partnered with chromosome 22 in the translocation leading to the aberrant production of BCR-ABL fusion protein often found in leukemias. The C9orf72 gene product has been provisionally designated C9orf72 pending further characterization. There are two isoforms of C9orf72 that are produced as a result of alternative splicing events. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is C9orf72 Polyclonal Antibody, PerCP-Cy5.5 Conjugated (BS-8595R-PERCP-CY5-5) validated for, and at what dilution?
BS-8595R-PERCP-CY5-5 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does C9orf72 Polyclonal Antibody, PerCP-Cy5.5 Conjugated (BS-8595R-PERCP-CY5-5) react with?
Tested reactivity: Human, Mouse, Rat.
How should BS-8595R-PERCP-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise C9orf72 Polyclonal Antibody, PerCP-Cy5.5 Conjugated (BS-8595R-PERCP-CY5-5)?
KLH conjugated synthetic peptide derived from human C9orf72
What are the excitation and emission wavelengths of the PerCP-Cy5.5 conjugate (BS-8595R-PERCP-CY5-5)?
PerCP-Cy5.5 on BS-8595R-PERCP-CY5-5: excitation / emission 482nm/694nm.