HLA-B Polyclonal Antibody, PE-Cy5.5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-8604R-PE-Cy5.5-100uL |
| Product Name | HLA-B Polyclonal Antibody, PE-Cy5.5 Conjugated |
| Applications |
WB
|
| Reactivity | Human |
| Specifications | |
| Conjugation | PE-Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human HLA B27 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Cell membrane |
| Synonyms | HLA B27 HLA-B27; HLAB; Leukocyte antigen class I B; Lymphocyte antigen; Major histocompatibility complex class I B; SPDA1; 1B27_HUMAN. |
| Background | Major histocompatibility complex (MHC) molecules form an integral part of the immune response system. They are cell-surface receptors that bind peptides and present them to T lymphocytes. Human leukocyte antigens (HLAs) are polymorphic members of the MHC family that are specifically involved in the presentation of antigens to the T cell receptor. There are two classes of HLA antigens: class I (HLA-A, HLA-B and HLA-C) and class II (HLA-D). Class I molecules are expressed in nearly all cells and play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum. The differential structural properties of MHC class I and class II molecules account for their respective roles in activating different populations of T lymphocytes. HLA-B encodes a membrane anchored heavy chain which hetero-dimerizes with a light chain (-2-Microglobulin) to form MHC-I. Polymorphisms yield hundreds of HLA-B alleles. The HLA-B27 allele appears with increased frequency in uveitis patients. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is HLA-B Polyclonal Antibody, PE-Cy5.5 Conjugated (BS-8604R-PE-CY5-5) validated for, and at what dilution?
BS-8604R-PE-CY5-5 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does HLA-B Polyclonal Antibody, PE-Cy5.5 Conjugated (BS-8604R-PE-CY5-5) react with?
Tested reactivity: Human.
How should BS-8604R-PE-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise HLA-B Polyclonal Antibody, PE-Cy5.5 Conjugated (BS-8604R-PE-CY5-5)?
KLH conjugated synthetic peptide derived from human HLA B27
What are the excitation and emission wavelengths of the PE-Cy5.5 conjugate (BS-8604R-PE-CY5-5)?
PE-Cy5.5 on BS-8604R-PE-CY5-5: excitation / emission 488nm/694nm.