USP22 Polyclonal Antibody, APC-Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-8611R-APC-Cy7-100uL |
| Product Name | USP22 Polyclonal Antibody, APC-Cy7 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Rat |
| Predicted Reactivity | Mouse, Dog, Cow, Sheep, Horse, Chicken, Rabbit, Xenopuslaevis |
| Specifications | |
| Conjugation | APC-Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human USP22 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 23326 |
| Swiss Prot | Q9UPT9 |
| Subcellular location | Nucleus |
| Synonyms | USP3L; Ubiquitin carboxyl-terminal hydrolase 22; Deubiquitinating enzyme 22; Ubiquitin thioesterase 22; Ubiquitin-specific-processing protease 22; USP22; KIAA1063 |
| Background | Histone deubiquitinating component of the transcription regulatory histone acetylation (HAT) complex SAGA. Catalyzes the deubiquitination of both histones H2A and H2B, thereby acting as a coactivator. Recruited to specific gene promoters by activators such as MYC, where it is required for transcription. Required for nuclear receptor-mediated transactivation and cell cycle progression. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is USP22 Polyclonal Antibody, APC-Cy7 Conjugated (bs-8611R-APC-Cy7) validated for, and at what dilution?
bs-8611R-APC-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does USP22 Polyclonal Antibody, APC-Cy7 Conjugated (bs-8611R-APC-Cy7) react with?
Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Dog, Cow, Sheep, Horse, Chicken, Rabbit, Xenopuslaevis.
How should bs-8611R-APC-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise USP22 Polyclonal Antibody, APC-Cy7 Conjugated (bs-8611R-APC-Cy7)?
KLH conjugated synthetic peptide derived from human USP22
What are the excitation and emission wavelengths of the APC-Cy7 conjugate (bs-8611R-APC-Cy7)?
APC-Cy7 on bs-8611R-APC-Cy7: excitation / emission 650nm/780nm.