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Complement C2 Polyclonal Antibody, Cy5.5 Conjugated

Applications

  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human

Predicted Reactivity

  • Mouse
  • Rat
  • Dog
  • Pig
  • Horse
Overview
Catalog # bs-8613R-Cy5.5-100uL
Product Name Complement C2 Polyclonal Antibody, Cy5.5 Conjugated
Applications
IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human
Predicted Reactivity Mouse, Rat, Dog, Pig, Horse
Specifications
Conjugation Cy5.5
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Complement C2a fragment
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 717
Swiss Prot P06681
Subcellular location Secreted
Synonyms CO2; ARMD14; Complement C2; C3/C5 convertase; C2
Background Component C2 which is part of the classical pathway of the complement system is cleaved by activated factor C1 into two fragments: C2b and C2a. C2a, a serine protease, then combines with complement factor C4b to generate the C3 or C5 convertase.
Application Dilution
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is Complement C2 Polyclonal Antibody, Cy5.5 Conjugated (BS-8613R-CY5-5) validated for, and at what dilution?

BS-8613R-CY5-5 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Complement C2 Polyclonal Antibody, Cy5.5 Conjugated (BS-8613R-CY5-5) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Pig, Horse.

How should BS-8613R-CY5-5 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Complement C2 Polyclonal Antibody, Cy5.5 Conjugated (BS-8613R-CY5-5)?

KLH conjugated synthetic peptide derived from human Complement C2a fragment

What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-8613R-CY5-5)?

Cy5.5 on BS-8613R-CY5-5: excitation / emission 675nm/694nm.