Cdc14A Polyclonal Antibody, RBITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-8614R-RBITC-100uL |
| Product Name | Cdc14A Polyclonal Antibody, RBITC Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat, Dog, Cow, Sheep, Horse |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Cdc14A |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 8556 |
| Swiss Prot | Q9UNH5 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | CDC 10; Cdc 14; Cdc 14A; Cdc 14A1; Cdc 14A2; CDC10; Cdc14; CDC14 cell division cycle 14 homolog A; CDC14 homolog A; Cdc14A1; Cdc14A2; Cell division cycle 14 homolog A; Dual specicity protein phosphatase CDC 14A; Dual specicity protein phosphatase CDC14A; hCDC14; CC14A_HUMAN. |
| Background | In budding yeast, the Cdc14a phosphatase activates mitotic exit by dephosphorylation of specific cyclin-dependent kinase (Cdk) substrates and seems to be regulated by sequestration in the nucleolus until its release during mitosis. Human Cdc14a phosphatase is highly similar to Saccharomyces cerevisiae Cdc14 and is a member of the dual specificity protein Tyrosine phosphatase family. It interacts with and dephosphorylates tumor suppressor protein p53 and may regulate the function of p53. In addition, Cdc14a dephosphorylates hCdh1 and activates APCCdh1. Cdc14a phosphatase plays a role in the regulation of the centrosome cycle, mitosis and cytokinesis, thereby influencing chromosome partitioning and genomic stability in human cells. Deregulated human Cdc14a phosphatase disrupts centrosome separation and chromosome segregation. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Cdc14A Polyclonal Antibody, RBITC Conjugated (bs-8614R-RBITC) validated for, and at what dilution?
bs-8614R-RBITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Cdc14A Polyclonal Antibody, RBITC Conjugated (bs-8614R-RBITC) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Cow, Sheep, Horse.
How should bs-8614R-RBITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Cdc14A Polyclonal Antibody, RBITC Conjugated (bs-8614R-RBITC)?
KLH conjugated synthetic peptide derived from human Cdc14A
What are the excitation and emission wavelengths of the RBITC conjugate (bs-8614R-RBITC)?
RBITC on bs-8614R-RBITC: excitation / emission 540nm/625nm.