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Ribonuclease 3/ECP Polyclonal Antibody, FITC Conjugated

Applications

  • IF(ICC)

Reactivity

  • Human
Overview
Catalog # bs-8615R-FITC
Product Name Ribonuclease 3/ECP Polyclonal Antibody, FITC Conjugated
Applications
IF(ICC)
Reactivity Human
Specifications
Conjugation FITC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human ECP
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 6037
Swiss Prot P12724
Subcellular location Cytoplasm
Synonyms ECP; RNS3; Eosinophil cationic protein; Ribonuclease 3; RNase 3; RNASE3
Background Cytotoxin and helminthotoxin with low-efficiency ribonuclease activity. Possesses a wide variety of biological activities. Exhibits antibacterial activity, including cytoplasmic membrane depolarization of preferentially Gram-negative, but also Gram-positive strains. Promotes E.coli outer membrane detachment, alteration of the overall cell shape and partial loss of cell content.
Application Dilution
IF(ICC) 1:50-200

Frequently asked questions

Which applications is Ribonuclease 3/ECP Polyclonal Antibody, FITC Conjugated (bs-8615R-FITC) validated for, and at what dilution?

bs-8615R-FITC is validated for IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Ribonuclease 3/ECP Polyclonal Antibody, FITC Conjugated (bs-8615R-FITC) react with?

Tested reactivity: Human.

How should bs-8615R-FITC be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Ribonuclease 3/ECP Polyclonal Antibody, FITC Conjugated (bs-8615R-FITC)?

KLH conjugated synthetic peptide derived from human ECP

What are the excitation and emission wavelengths of the FITC conjugate (bs-8615R-FITC)?

FITC on bs-8615R-FITC: excitation / emission 494nm/518nm.