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GPR7 Polyclonal Antibody, AbBy Fluor-405 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Mouse

Predicted Reactivity

  • Human
Overview
Catalog # bs-8618R-BF405-100uL
Product Name GPR7 Polyclonal Antibody, AbBy Fluor-405 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Mouse
Predicted Reactivity Human
Specifications
Conjugation AbBy Fluor-405
Host Rabbit
Source KLH conjugated synthetic peptide derived from human GPR7
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 2831
Subcellular location Cell membrane
Synonyms GPCR GPR7; G protein coupled receptor 7; g protein coupled receptor gpr7; GPR7; MGC129755; Neuropeptides B and W receptor 1; Neuropeptides B/W receptor 1; Neuropeptides B/W receptor type 1; NPB and NPW receptor 1; NPBWR1; opioid somatostatin like receptor 7.
Background The two G protein-coupled receptors GPR7 and GPR8 display high similarity to each other. They both show high expression in brain and in particular in hypothalamus, and have been characterized as receptors for neuropeptide W (NPW) and neuropeptide B (NPB). In response to NPW and NPB, they play a role in the regulation of feeding behavior. GPR7 deficient mice develop an adult-onset obese phenotype that progressively worsens with age and is exacerbated when fed a high-fat diet. The genes encoding human GPR7 and GPR8 map to chromosomes 10q11.2-q21.1 and 10q13.3, respectively.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is GPR7 Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-8618R-BF405) validated for, and at what dilution?

bs-8618R-BF405 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does GPR7 Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-8618R-BF405) react with?

Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human.

How should bs-8618R-BF405 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise GPR7 Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-8618R-BF405)?

KLH conjugated synthetic peptide derived from human GPR7

What are the excitation and emission wavelengths of the AbBy Fluor-405 conjugate (bs-8618R-BF405)?

AbBy Fluor-405 on bs-8618R-BF405: excitation / emission 401nm/421nm.

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