PDE3B Polyclonal Antibody, Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-8621R-Cy7-100uL |
| Product Name | PDE3B Polyclonal Antibody, Cy7 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human PDE3B |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5140 |
| Swiss Prot | Q13370 |
| Subcellular location | Cell membrane |
| Synonyms | 5''-cyclic phosphodiesterase B; Adipocyte cAMP phosphodiesterase cGMP inhibited; AI847709; cAMP Phosphodiesterase, adipocyte, cGMP-inhibited; CGI-PDE B; CGIP1; CGIPDE B; CGIPDE1; cGMP inhibited 3 5 cyclic phosphodiesterase B; CGMP inhibited cAMP phophodiesterase; cGMP-inhibited 3''; Cyclic GMP inhibited phosphodiesterase B; Cyclic GMP-inhibited phosphodiesterase B; Cyclic nucleotide phosphodiesterase; HcGIP1; HcGIP1, rat, homolog of; Pde3b; PDE3B_HUMAN; Phosphodiesterase 3B cGMP inhibited. |
| Background | PDE3B is found in lymphocytes. The PDE7A1 (HCP1) isozyme and the PDE7A2 proteins, alternate splice products of PDE7A, are highly expressed in skeletal muscle. PDE7B is most highly expressed in pancreas. The PDE family contains proteins that serve tissue-specific roles in regulation of lipolysis, glycogenolysis, myocardial contractility, and smooth muscle relaxation. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is PDE3B Polyclonal Antibody, Cy7 Conjugated (bs-8621R-Cy7) validated for, and at what dilution?
bs-8621R-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PDE3B Polyclonal Antibody, Cy7 Conjugated (bs-8621R-Cy7) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Pig, Horse, Rabbit.
How should bs-8621R-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PDE3B Polyclonal Antibody, Cy7 Conjugated (bs-8621R-Cy7)?
KLH conjugated synthetic peptide derived from human PDE3B
What are the excitation and emission wavelengths of the Cy7 conjugate (bs-8621R-Cy7)?
Cy7 on bs-8621R-Cy7: excitation / emission 743nm/767nm.