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TESPA1 Polyclonal Antibody, PE Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human

Predicted Reactivity

  • Rabbit
Overview
Catalog # bs-8623R-PE
Product Name TESPA1 Polyclonal Antibody, PE Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human
Predicted Reactivity Rabbit
Specifications
Conjugation PE
Host Rabbit
Source KLH conjugated synthetic peptide derived from human TESPA1
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 9840
Swiss Prot A2RU30
Subcellular location Cytoplasm
Synonyms Protein TESPA1; TESPA1; Thymocyte-expressed positive selection-associated protein 1; KIAA0748; HSPC257
Background Required for the development and maturation of T-cells, its function being essential for the late stages of thymocyte development (By similarity). Plays a role in T-cell antigen receptor (TCR)-mediated activation of the ERK and NFAT signaling pathways, possibly by serving as a scaffolding protein that promotes the assembly of the LAT signalosome in thymocytes. May play a role in the regulation of inositol 1,4,5-trisphosphate receptor-mediated Ca2+ release and mitochondrial Ca2+ uptake via the mitochondria-associated endoplasmic reticulum membrane (MAM) compartment.
Application Dilution
WB 1:300-5000
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Frequently asked questions

Which applications is TESPA1 Polyclonal Antibody, PE Conjugated (bs-8623R-PE) validated for, and at what dilution?

bs-8623R-PE is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does TESPA1 Polyclonal Antibody, PE Conjugated (bs-8623R-PE) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Rabbit.

How should bs-8623R-PE be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise TESPA1 Polyclonal Antibody, PE Conjugated (bs-8623R-PE)?

KLH conjugated synthetic peptide derived from human TESPA1

What are the excitation and emission wavelengths of the PE conjugate (bs-8623R-PE)?

PE on bs-8623R-PE: excitation / emission 496,564nm/578nm.