T2R38 Polyclonal Antibody, AbBy Fluor-647 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-8650R-BF647-100uL |
| Product Name | T2R38 Polyclonal Antibody, AbBy Fluor-647 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Chicken |
| Specifications | |
| Conjugation | AbBy Fluor-647 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human T2R38/TAS2R38 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5726 |
| Subcellular location | Cell membrane |
| Synonyms | PTC; PTC bitter taste receptor; T2R38; T2R61; taste receptor, type 2, member 38; T2R38_HUMAN. |
| Background | The sense of taste is essential for the survival of organisms. For example, the ability to identify sweet-tasting foods enables animals to seek out food with high nutritive value, whereas the ability to identify bitter substances enables them to avoid the ingestion of potentially harmful substances. A family of integral membrane proteins are involved in taste perception and include T1R, which is involved in sweet taste perception and T2R, which is involved in bitter taste perception. Both types of taste receptors couple to various G proteins to initiate signal transduction cascades. Specifically, T2R38 is expressed in subsets of taste receptor cells of the tongue and exclusively in gustducin-positive cells. Variations in T2R38 are associated with the ability to taste the bitter chemical phenylthiocarbamide (PTC), also called thiourea tasting. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is T2R38 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-8650R-BF647) validated for, and at what dilution?
bs-8650R-BF647 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-8650R-BF647 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise T2R38 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-8650R-BF647)?
KLH conjugated synthetic peptide derived from human T2R38/TAS2R38
What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bs-8650R-BF647)?
AbBy Fluor-647 on bs-8650R-BF647: excitation / emission 650nm/665nm.