phospho-ZIP Kinase (Thr265) Polyclonal Antibody, AbBy Fluor-488 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-8664R-BF488 |
| Product Name | phospho-ZIP Kinase (Thr265) Polyclonal Antibody, AbBy Fluor-488 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Dog, Cow, Pig, Horse |
| Specifications | |
| Conjugation | AbBy Fluor-488 |
| Host | Rabbit |
| Source | KLH conjugated synthesised phosphopeptide derived from human ZIP Kinase around the phosphorylation site of Thr265 |
| Modification Site | Thr265 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1613 |
| Swiss Prot | O43293 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | Death-associated protein kinase 3; DAPK3; DAP kinase 3; Dlk; MYPT1 kinase; Zipper-interacting protein kinase; ZIP-kinase; ZIPK |
| Background | Serine/threonine kinase which acts as a positive regulator of apoptosis. Phosphorylates histone H3 on 'Thr-11' at centromeres during mitosis. Regulates myosin light chain phosphatase through phosphorylation of MYPT1 thereby regulating the assembly of the actin cytoskeleton, cell migration, invasiveness of tumor cells, smooth muscle contraction and neurite outgrowth. Involved in the formation of promyelocytic leukemia protein nuclear body (PML-NB), one of many subnuclear domains in the eukaryotic cell nucleus, and which is involved in oncogenesis and viral infection. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-ZIP Kinase (Thr265) Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-8664R-BF488) validated for, and at what dilution?
bs-8664R-BF488 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-ZIP Kinase (Thr265) Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-8664R-BF488) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Cow, Pig, Horse.
How should bs-8664R-BF488 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-ZIP Kinase (Thr265) Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-8664R-BF488)?
KLH conjugated synthesised phosphopeptide derived from human ZIP Kinase around the phosphorylation site of Thr265
What are the excitation and emission wavelengths of the AbBy Fluor-488 conjugate (bs-8664R-BF488)?
AbBy Fluor-488 on bs-8664R-BF488: excitation / emission 499nm/519nm.