phospho-IRS1 (Tyr1179) Polyclonal Antibody, Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-8709R-Cy7-100uL |
| Product Name | phospho-IRS1 (Tyr1179) Polyclonal Antibody, Cy7 Conjugated |
| Applications |
FCM
IF(ICC)
IF
|
| Reactivity | Human, Mouse, Zebrafish |
| Predicted Reactivity | Rat, Dog, Cow, Sheep, Pig, Chicken, Rabbit, GuineaPig |
| Specifications | |
| Conjugation | Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthesised phosphopeptide derived from human IRS1 around the phosphorylation site of Ser1179. |
| Modification Site | Tyr1179 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3667 |
| Swiss Prot | P35568 |
| Subcellular location | Cell membrane |
| Synonyms | IRS1 (phospho S1179); p-IRS1 (phospho S1179); p-IRS1; IRS1(phospho-Ser1179); HIRS 1; HIRS1; Insulin Receptor Substrate 1; IRS-1; IRS 1; IRS1; OTTHUMP00000164234; IRS1_HUMAN. |
| Background | May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit. |
| Application Dilution | |
| FCM | 1:20-100 |
| IF(ICC) | 1:50-200 |
| IF | |
Frequently asked questions
Which applications is phospho-IRS1 (Tyr1179) Polyclonal Antibody, Cy7 Conjugated (bs-8709R-Cy7) validated for, and at what dilution?
bs-8709R-Cy7 is validated for FCM (1:20-100); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-IRS1 (Tyr1179) Polyclonal Antibody, Cy7 Conjugated (bs-8709R-Cy7) react with?
Tested reactivity: Human, Mouse, Zebrafish. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Cow, Sheep, Pig, Chicken, Rabbit, GuineaPig.
How should bs-8709R-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-IRS1 (Tyr1179) Polyclonal Antibody, Cy7 Conjugated (bs-8709R-Cy7)?
KLH conjugated synthesised phosphopeptide derived from human IRS1 around the phosphorylation site of Ser1179.
What are the excitation and emission wavelengths of the Cy7 conjugate (bs-8709R-Cy7)?
Cy7 on bs-8709R-Cy7: excitation / emission 743nm/767nm.