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phospho-IRS1 (Tyr1179) Polyclonal Antibody

HepG2 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with IRS1 (Tyr1179) Polyclonal Antibody(bs-8709R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
HepG2 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with IRS1 (Tyr1179) Polyclonal Antibody(bs-8709R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Bioss unconjugated antibody vial

Applications

  • ELISA
  • FCM
  • IHC-P
  • IHC-F
  • IF(ICC)
  • IF

Reactivity

  • Human
  • Mouse
  • Zebrafish

Predicted Reactivity

  • Rat
  • Dog
  • Cow
  • Sheep
  • Pig
  • Chicken
  • Rabbit
  • GuineaPig
Overview
Catalog # bs-8709R
Product Name phospho-IRS1 (Tyr1179) Polyclonal Antibody
Applications
ELISA FCM IHC-P IHC-F IF(ICC) IF
Reactivity Human, Mouse, Zebrafish
Predicted Reactivity Rat, Dog, Cow, Sheep, Pig, Chicken, Rabbit, GuineaPig
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthesised phosphopeptide derived from human IRS1 around the phosphorylation site of Ser1179.
Modification Site Tyr1179
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 3667
Swiss Prot P35568
Subcellular location Cell membrane
Synonyms IRS1 (phospho S1179); p-IRS1 (phospho S1179); p-IRS1; IRS1(phospho-Ser1179); HIRS 1; HIRS1; Insulin Receptor Substrate 1; IRS-1; IRS 1; IRS1; OTTHUMP00000164234; IRS1_HUMAN.
Background May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit.
Application Dilution
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(ICC) 1:50-200
IF

Frequently asked questions

Which applications is phospho-IRS1 (Tyr1179) Polyclonal Antibody (bs-8709R) validated for, and at what dilution?

bs-8709R is validated for ELISA (1:500-1000); FCM (1:20-100); IHC-P (1:200-400); IHC-F (1:100-500); IF(ICC) (1:50-200); IF. These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does phospho-IRS1 (Tyr1179) Polyclonal Antibody (bs-8709R) react with?

Tested reactivity: Human, Mouse, Zebrafish. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Cow, Sheep, Pig, Chicken, Rabbit, GuineaPig.

How should bs-8709R be stored?

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise phospho-IRS1 (Tyr1179) Polyclonal Antibody (bs-8709R)?

KLH conjugated synthesised phosphopeptide derived from human IRS1 around the phosphorylation site of Ser1179.

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