phospho-IRS1 (Tyr1179) Polyclonal Antibody, Biotin Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-8709R-Biotin-100uL |
| Product Name | phospho-IRS1 (Tyr1179) Polyclonal Antibody, Biotin Conjugated |
| Applications |
ELISA
IHC-P
IHC-F
|
| Reactivity | Human, Mouse, Zebrafish |
| Predicted Reactivity | Rat, Dog, Cow, Sheep, Pig, Chicken, Rabbit, GuineaPig |
| Specifications | |
| Conjugation | Biotin |
| Host | Rabbit |
| Source | KLH conjugated synthesised phosphopeptide derived from human IRS1 around the phosphorylation site of Ser1179. |
| Modification Site | Tyr1179 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C for 12 months. |
| Target | |
| Gene ID | 3667 |
| Swiss Prot | P35568 |
| Subcellular location | Cell membrane |
| Synonyms | IRS1 (phospho S1179); p-IRS1 (phospho S1179); p-IRS1; IRS1(phospho-Ser1179); HIRS 1; HIRS1; Insulin Receptor Substrate 1; IRS-1; IRS 1; IRS1; OTTHUMP00000164234; IRS1_HUMAN. |
| Background | May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit. |
| Application Dilution | |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
Frequently asked questions
Which applications is phospho-IRS1 (Tyr1179) Polyclonal Antibody, Biotin Conjugated (bs-8709R-Biotin) validated for, and at what dilution?
bs-8709R-Biotin is validated for ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-IRS1 (Tyr1179) Polyclonal Antibody, Biotin Conjugated (bs-8709R-Biotin) react with?
Tested reactivity: Human, Mouse, Zebrafish. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Cow, Sheep, Pig, Chicken, Rabbit, GuineaPig.
How should bs-8709R-Biotin be stored?
Store at -20C for 12 months. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-IRS1 (Tyr1179) Polyclonal Antibody, Biotin Conjugated (bs-8709R-Biotin)?
KLH conjugated synthesised phosphopeptide derived from human IRS1 around the phosphorylation site of Ser1179.