ANKRD6 Polyclonal Antibody, PE-Cy5 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-8728R-PE-Cy5-100uL |
| Product Name | ANKRD6 Polyclonal Antibody, PE-Cy5 Conjugated |
| Applications |
WB
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Sheep, Horse |
| Specifications | |
| Conjugation | PE-Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ANKRD6/Diversin |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Synonyms | ANKRD 6; ankyrin repeat domain 6; ANKR6_HUMAN; Ankyrin repeat domain-containing protein 6; Diversin. |
| Background | Diversin, also known as ANKRD6 (ankyrin repeat domain 6), is a 727 amino acid protein that contains eight ankyrin repeats and belongs to the ankyrin repeat domain protein family. Members of the ankyrin repeat domain family facilitate protein-protein interactions and function as adaptors of signaling pathways. Expressed in a developmentally-regulated manner and at highest levels in the brain, Diversin is believed to play a role in brain development. Via its ankyrin repeats, Diversin can directly interact with Dvl (dishevelled), an interaction that is essential for the activation of noncanonical Wnt signaling. In addition, Diversin contains a C-terminal domain that binds Axin/Conductin and a casein kinase-binding domain in its central region that specifically binds casein kinase I?. Through the action of these additional domains, Diversin may also facilitate canonical Wnt signaling. Due to alternative splicing events, three Diversin isoforms exist. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is ANKRD6 Polyclonal Antibody, PE-Cy5 Conjugated (bs-8728R-PE-Cy5) validated for, and at what dilution?
bs-8728R-PE-Cy5 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-8728R-PE-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ANKRD6 Polyclonal Antibody, PE-Cy5 Conjugated (bs-8728R-PE-Cy5)?
KLH conjugated synthetic peptide derived from human ANKRD6/Diversin
What are the excitation and emission wavelengths of the PE-Cy5 conjugate (bs-8728R-PE-Cy5)?
PE-Cy5 on bs-8728R-PE-Cy5: excitation / emission 488nm/670nm.