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WIPI2/Atg21 Polyclonal Antibody, RBITC Conjugated

Applications

  • WB

Reactivity

  • Mouse
  • Rat

Predicted Reactivity

  • Human
  • Dog
  • Cow
  • Pig
  • Horse
  • Chicken
Overview
Catalog # bs-8767R-RBITC
Product Name WIPI2/Atg21 Polyclonal Antibody, RBITC Conjugated
Applications
WB
Reactivity Mouse, Rat
Predicted Reactivity Human, Dog, Cow, Pig, Horse, Chicken
Specifications
Conjugation RBITC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human WIPI2/Atg21
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Subcellular location Cytoplasm
Synonyms ATG18B; Atg21; CGI 50; DKFZp434J154; DKFZp686P02188; FLJ12979; FLJ14217; FLJ42984; WD repeat domain phosphoinositide-interacting protein 2; WD repeat domain, phosphoinositide interacting 2; WD40 repeat protein interacting with phosphoinositides 2; WIPI 2; WIPI-2; WIPI2; WIPI2_HUMAN; WIPI49 like protein 2; WIPI49-like protein 2.
Background Probable early component of the autophagy machinery being involved in formation of preautophagosomal structures and their maturation into mature phagosomes in response to PtdIns3P. May bind PtdIns3P.
Application Dilution
WB =1:500-2000

Frequently asked questions

Which applications is WIPI2/Atg21 Polyclonal Antibody, RBITC Conjugated (bs-8767R-RBITC) validated for, and at what dilution?

bs-8767R-RBITC is validated for WB (WB=1:500-2000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does WIPI2/Atg21 Polyclonal Antibody, RBITC Conjugated (bs-8767R-RBITC) react with?

Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Cow, Pig, Horse, Chicken.

How should bs-8767R-RBITC be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise WIPI2/Atg21 Polyclonal Antibody, RBITC Conjugated (bs-8767R-RBITC)?

KLH conjugated synthetic peptide derived from human WIPI2/Atg21

What are the excitation and emission wavelengths of the RBITC conjugate (bs-8767R-RBITC)?

RBITC on bs-8767R-RBITC: excitation / emission 540nm/625nm.