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HMG4/HMGB3 Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded Rat bladder; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with HMG4\/HMGB3 Polyclonal Antibody, Unconjugated (bs-8809R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat bladder; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with HMG4\/HMGB3 Polyclonal Antibody, Unconjugated (bs-8809R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse bladder; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with HMG4\/HMGB3 Polyclonal Antibody, Unconjugated (bs-8809R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse bladder; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with HMG4\/HMGB3 Polyclonal Antibody, Unconjugated (bs-8809R) at 1:200 overnight at 4\u00b0C, DAB staining.
Bioss unconjugated antibody vial

Applications

  • IHC-P
  • IHC-F
  • IF

Reactivity

  • Mouse
  • Rat
Overview
Catalog # bs-8809R
Product Name HMG4/HMGB3 Polyclonal Antibody
Applications
IHC-P IHC-F IF
Reactivity Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human HMG4/HMGB3:1-100/200
Immunogen Range 1-100/200
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 3149
Swiss Prot O15347
Subcellular location Cytoplasm, Nucleus, Chromosome
Synonyms High mobility group (nonhistone chromosomal) protein 4; High mobility group box 3; High mobility group protein 2a; High mobility group protein 4; High mobility group protein B3; High mobility group protein HMG4; HMG 4; HMG-2a; HMG-4; HMG2A; HMGB 3; HMGB3; HMGB3_HUMAN; MGC90319; Non histone chromosomal protein; Nonhistone chromosomal protein HMG4.
Background HMGB3 belongs to the high mobility group (HMG) protein superfamily. Like HMG1 (MIM 163905) and HMG2 (MIM 163906), HMGB3 contains DNA-binding HMG box domains and is classified into the HMG box subfamily. Members of the HMG box subfamily are thought to play a fundamental role in DNA replication, nucleosome assembly and transcription (Wilke et al., 1997 [PubMed 9370291]; Nemeth et al., 2006 [PubMed 16945912]).[supplied by OMIM, Mar 2008] Function : Binds preferentially single-stranded DNA and unwinds double stranded DNA. Similarity : Belongs to the HMGB family. Contains 2 HMG box DNA-binding domains.
Application Dilution
IHC-P 1:200-400
IHC-F
IF

Frequently asked questions

Which applications is HMG4/HMGB3 Polyclonal Antibody (bs-8809R) validated for, and at what dilution?

bs-8809R is validated for IHC-P (1:200-400). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does HMG4/HMGB3 Polyclonal Antibody (bs-8809R) react with?

Tested reactivity: Mouse, Rat.

How should bs-8809R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise HMG4/HMGB3 Polyclonal Antibody (bs-8809R)?

KLH conjugated synthetic peptide derived from human HMG4/HMGB3:1-100/200 Immunogen range: 1-100/200.

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