SMAD8/SMAD9 Polyclonal Antibody, HRP Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-8844R-HRP-100uL |
| Product Name | SMAD8/SMAD9 Polyclonal Antibody, HRP Conjugated |
| Applications |
ELISA
IHC-P
IHC-F
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Zebrafish |
| Specifications | |
| Conjugation | HRP |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human SMAD8 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 142891 |
| Swiss Prot | Q96LT4 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | AI528653 ; BSP 1 ; BSP1 ; DKFZp781C1895 ; DKFZp781O1323 ; Dwarfin A ; Dwf A ; DwfA ; Dwfc ; hSMAD1 ; hSmad5 ; JV 41 ; JV4 1 ; JV41 ; JV5 1 ; MAD (mothers against decapentaplegic, Drosophila) homolog 1 ; MAD (mothers against decapentaplegic, Drosophila) homolog 9 ; MAD homolog 1 ; MAD homolog 9 ; MAD homolog1 (mothers against decapentaplegic, Drosophila) ; |
| Background | Sphingomyelin synthases synthesize sphingolipids through transfer of a phosphatidyl head group on to the primary hydroxyl of ceramide. SAMD8 is an endoplasmic reticulum (ER) transferase that has no sphingomyelin synthase activity but can convert phosphatidylethanolamine (PE) and ceramide to ceramide phosphoethanolamine (CPE) albeit with low product yield. Appears to operate as a ceramide sensor to control ceramide homeostasis in the endoplasmic reticulum rather than a converter of ceramides. Seems to be critical for the integrity of the early secretory pathway |
| Application Dilution | |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
Frequently asked questions
Which applications is SMAD8/SMAD9 Polyclonal Antibody, HRP Conjugated (bs-8844R-HRP) validated for, and at what dilution?
bs-8844R-HRP is validated for ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does SMAD8/SMAD9 Polyclonal Antibody, HRP Conjugated (bs-8844R-HRP) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Zebrafish.
How should bs-8844R-HRP be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise SMAD8/SMAD9 Polyclonal Antibody, HRP Conjugated (bs-8844R-HRP)?
KLH conjugated synthetic peptide derived from human SMAD8