phospho-Smad2/Smad3 (Thr8) Polyclonal Antibody, APC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-8853R-APC |
| Product Name | phospho-Smad2/Smad3 (Thr8) Polyclonal Antibody, APC Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(ICC)
IF
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat, Dog, Cow, Pig, Horse, Chicken |
| Specifications | |
| Conjugation | APC |
| Host | Rabbit |
| Source | KLH conjugated synthesised phosphopeptide derived from human Smad2/Smad3 around the phosphorylation site of Thr8 |
| Modification Site | Thr8 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4087 |
| Swiss Prot | P84022 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | Smad2 + Smad3 (phospho T8); p-Smad2 + Smad3 (phospho T8); P-Smad2 /3 (phospho T8); hMAD 2; hMAD 3; hSMAD2; hSMAD3; Mad related protein 2; MADH2; MADH3; MADR2; Mothers against DPP homolog 2; Mothers against DPP homolog 3; Sma and Mad related protein 2; SMA and MAD related protein 3; SMAD 2; SMAD 3; SMAD family member 2; SMAD family member 3. |
| Background | SMAD is a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. They mediate the signal of the transforming growth factor (TGF)-beta, and thus regulate multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(ICC) | 1:50-200 |
| IF | |