KPNA1 Polyclonal Antibody, APC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-8864R-APC-100uL |
| Product Name | KPNA1 Polyclonal Antibody, APC Conjugated |
| Applications |
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | APC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human SRP1/karyopherin alpha 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3838 |
| Synonyms | IMA1_HUMAN; Importin alpha 1 subunit; Importin alpha 5; Importin alpha S1; Importin subunit alpha-1; IPO A5; IPOA 5; IPOA5; Karyopherin alpha 1; Karyopherin alpha 1 subunit; Karyopherin subunit alpha-1; KPNA 1; KPNA1; mSRP 1; mSRP1; NPI 1; NPI-1; NPI1; Nucleoprotein interactor 1; RAG cohort protein 2; RCH 2; RCH2; Recombination activating gene cohort 2; SRP 1; SRP1 beta; SRP1-beta. |
| Background | Protein transport across the nucleus is a selective, multi-step process involving several cytoplasmic factors that mediate protein passage through the nuclear pore complex (NPC). Cytoplasmic proteins that contain nuclear localization signals (NLSs) must be recognized as import substrates, dock at the nuclear pore complex and translocate across the nuclear envelope in an ATP-dependent fashion. Karyopherin alpha 1 and karyopherin alpha 6 are widely expressed nuclear import proteins that act as adaptors for karyopherin 1, specifically binding to and guiding NLS-containing proteins to the NPC. Both karyopherin alpha 1 and karyopherin alpha 6 contain one IBB domain and ten ARM repeats through which they convey their protein binding and localization function. Together, karyopherin å1 and karyopherin å6 are responsible for ensuring the nuclear import of NLS-containing substrates |
| Application Dilution | |
| IF | WB1:300-5000 |
Frequently asked questions
Which applications is KPNA1 Polyclonal Antibody, APC Conjugated (bs-8864R-APC) validated for, and at what dilution?
bs-8864R-APC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does KPNA1 Polyclonal Antibody, APC Conjugated (bs-8864R-APC) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-8864R-APC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise KPNA1 Polyclonal Antibody, APC Conjugated (bs-8864R-APC)?
KLH conjugated synthetic peptide derived from human SRP1/karyopherin alpha 1
What are the excitation and emission wavelengths of the APC conjugate (bs-8864R-APC)?
APC on bs-8864R-APC: excitation / emission 650nm/660nm.