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CD28 Polyclonal Antibody, AbBy Fluor-405 Conjugated

Applications

  • WB
  • IF(IHC-P)

Reactivity

  • Human
  • Mouse

Predicted Reactivity

  • Rat
Overview
Catalog # bs-8865R-BF405
Product Name CD28 Polyclonal Antibody, AbBy Fluor-405 Conjugated
Applications
WB IF(IHC-P)
Reactivity Human, Mouse
Predicted Reactivity Rat
Specifications
Conjugation AbBy Fluor-405
Host Rabbit
Source KLH conjugated synthetic peptide derived from human CD28
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 940
Swiss Prot P10747
Subcellular location Extracellular
Synonyms Tp44; T-cell-specific surface glycoprotein CD28; CD28
Background Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200

Frequently asked questions

Which applications is CD28 Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-8865R-BF405) validated for, and at what dilution?

bs-8865R-BF405 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does CD28 Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-8865R-BF405) react with?

Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat.

How should bs-8865R-BF405 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise CD28 Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-8865R-BF405)?

KLH conjugated synthetic peptide derived from human CD28

What are the excitation and emission wavelengths of the AbBy Fluor-405 conjugate (bs-8865R-BF405)?

AbBy Fluor-405 on bs-8865R-BF405: excitation / emission 401nm/421nm.