PISD Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-8866R-Cy3-100uL |
| Product Name | PISD Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat, Dog, Pig, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human PISD |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 23761 |
| Synonyms | DJ858B16; dJ858B16.2; DKFZp566G2246; Phosphatidylserine decarboxylase; Phosphatidylserine decarboxylase beta chain; Phosphatidylserine decarboxylase proenzyme; PISD; PISD_HUMAN; PSD; PSDC; PSSC. |
| Background | Enzymes known as phosphatidylserine decarboxylases (PSDs) catalyze the formation of phosphatidylethanolamine from phosphatidylserine via phosphatidylserine decarboxylation. Type I PSDs contain LGST motifs and are found in bacteria and eukaryotic mitochondria, whereas type II PSDs contain GGST motifs and are found in eukaryotic endomembrane systems. PISD (phosphatidylserine decarboxylase), also known as phosphatidylserine decarboxylase proenzyme, PSDC, PSD, PSSC, DJ858B16, dJ858B16.2 or DKFZp566G2246, is a 408 amino acid a type I phosphatidylserine decarboxylase that localizes to the inner mitochondrial membrane. PISD contains a conserved LGST motif which is cleaved to produce two isoforms known as PISD and PISD _. PISD is capable of forming a heterodimer and is highly expressed in liver and testis. The gene encoding PISD maps to human chromosome 22q12.2. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is PISD Polyclonal Antibody, Cy3 Conjugated (bs-8866R-Cy3) validated for, and at what dilution?
bs-8866R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PISD Polyclonal Antibody, Cy3 Conjugated (bs-8866R-Cy3) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Pig, Horse, Chicken, Rabbit.
How should bs-8866R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PISD Polyclonal Antibody, Cy3 Conjugated (bs-8866R-Cy3)?
KLH conjugated synthetic peptide derived from human PISD
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-8866R-Cy3)?
Cy3 on bs-8866R-Cy3: excitation / emission 512,550nm/570,615nm.