GHRHR Polyclonal Antibody, AbBy Fluor-555 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-8867R-BF555-100uL |
| Product Name | GHRHR Polyclonal Antibody, AbBy Fluor-555 Conjugated |
| Applications |
WB
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-555 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human GHRHR |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 2692 |
| Synonyms | GHRFR; GHRH R; GHRH receptor; Ghrhr; GHRHR_HUMAN; GHRHRpsv; GRF R; GRF receptor; GRFR; Growth hormone releasing factor receptor; Growth hormone releasing hormone receptor; Growth hormone-releasing factor receptor; Growth hormone-releasing hormone receptor. |
| Background | GHRH-R is a seven transmembrane domain protein that localizes to the somatotroph of the pituitary. GHRH-R plays an important role in growth and acts as a high-affinity receptor for GHRH. Binding of GHRH leads to the coupling of GHRH-R to G-protein which stimulates increased adenylyl cyclase activity and the accumulation of cAMP leading to the synthesis and release of growth hormone and somatotroph proliferation. In addition, this signalling pathway may have direct action in fetal/placental development, reproduction and immune function. GHRH and GHRH-R may also play a role in the regulation of non-rapid eye movement sleep (NREMS). The expression of GHRH-R is dependent on the presence of the POU domain factor Pit-1. Mutations in the gene encoding this protein can result in isolated growth hormone deficiency (IGHD), also known as Dwarfism of Sindh, and anterior pituitary hypoplasia (APH). |
| Application Dilution | |
| WB | 1:300-5000 |
| IF | IHC-P1:50-200 |
Frequently asked questions
Which applications is GHRHR Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-8867R-BF555) validated for, and at what dilution?
bs-8867R-BF555 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does GHRHR Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-8867R-BF555) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-8867R-BF555 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise GHRHR Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-8867R-BF555)?
KLH conjugated synthetic peptide derived from human GHRHR
What are the excitation and emission wavelengths of the AbBy Fluor-555 conjugate (bs-8867R-BF555)?
AbBy Fluor-555 on bs-8867R-BF555: excitation / emission 553nm/568nm.