Hepcidin 25 Polyclonal Antibody, APC-Cy7 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-8870R-APC-Cy7-100uL |
| Product Name | Hepcidin 25 Polyclonal Antibody, APC-Cy7 Conjugated |
| Applications |
IF(IHC-P)
|
| Reactivity | Mouse, Rat |
| Specifications | |
| Conjugation | APC-Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from rat Hepcidin |
| Immunogen Range | 60-84/84 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 84604 |
| Swiss Prot | Q99MH3 |
| Synonyms | Hamp; HEPC; HEPC_RAT; HEPC_MOUSE; Hepc20; Hepc25; HEPCIDIN; Hepcidin; Hepcidin 20; Hepcidin 25; Hepcidin antimicrobial peptide; Hepcidin-20; Hepcidin25; HFE2; HFE2B; LEAP 1; LEAP-1; LEAP1; Liver expressed antimicrobial peptide; Liver-expressed antimicrobial peptide 1; PLTR; Putative liver tumor regressor. |
| Background | The product encoded by this gene is involved in the maintenance of iron homeostasis, and it is necessary for the regulation of iron storage in macrophages, and for intestinal iron absorption. The preproprotein is post-translationally cleaved into mature peptides of 20, 22 and 25 amino acids, and these active peptides are rich in cysteines, which form intramolecular bonds that stabilize their beta-sheet structures. These peptides exhibit antimicrobial activity. Mutations in this gene cause hemochromatosis type 2B, also known as juvenile hemochromatosis, a disease caused by severe iron overload that results in cardiomyopathy, cirrhosis, and endocrine failure. [provided by RefSeq, Jul 2008]. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is Hepcidin 25 Polyclonal Antibody, APC-Cy7 Conjugated (bs-8870R-APC-Cy7) validated for, and at what dilution?
bs-8870R-APC-Cy7 is validated for IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Hepcidin 25 Polyclonal Antibody, APC-Cy7 Conjugated (bs-8870R-APC-Cy7) react with?
Tested reactivity: Mouse, Rat.
How should bs-8870R-APC-Cy7 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Hepcidin 25 Polyclonal Antibody, APC-Cy7 Conjugated (bs-8870R-APC-Cy7)?
KLH conjugated synthetic peptide derived from rat Hepcidin Immunogen range: 60-84/84.
What are the excitation and emission wavelengths of the APC-Cy7 conjugate (bs-8870R-APC-Cy7)?
APC-Cy7 on bs-8870R-APC-Cy7: excitation / emission 650nm/780nm.