CYP2D6 Polyclonal Antibody, Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-8872R-Cy7 |
| Product Name | CYP2D6 Polyclonal Antibody, Cy7 Conjugated |
| Applications |
WB
FCM
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Horse, Rabbit |
| Specifications | |
| Conjugation | Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CYP2D6 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | P10635 |
| Swiss Prot | 1565 |
| Synonyms | Cytochrome P450 2D6; CPD6; CYP2D; CYP2D6; CYP2DL1; CYPIID6; Cytochrome P450 family 2 subfamily D polypeptide 6; Debrisoquine 4 hydroxylase; EC 1.14.14.1; Flavoprotein linked monooxygenase; MGC120389; MGC120390; Microsomal monooxygenase; P450 DB1; P450C2D; P450DB1; Xenobiotic monooxygenase; CP2D6_HUMAN; Cytochrome P450-DB1; Debrisoquine 4-hydroxylase. |
| Background | This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is known to metabolize as many as 25% of commonly prescribed drugs. Its substrates include antidepressants, antipsychotics, analgesics and antitussives, beta adrenergic blocking agents, antiarrythmics and antiemetics. The gene is highly polymorphic in the human population; certain alleles result in the poor metabolizer phenotype, characterized by a decreased ability to metabolize the enzyme's substrates. Some individuals with the poor metabolizer phenotype have no functional protein since they carry 2 alleles whereas in other individuals the gene is absent. This gene can vary in copy number and individuals with the ultrarapid metabolizer phenotype can have 3 or more active copies of the gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]. |
| Application Dilution | |
| WB | 1:500-2000 |
| FCM | 1:100 |
Frequently asked questions
Which applications is CYP2D6 Polyclonal Antibody, Cy7 Conjugated (bs-8872R-Cy7) validated for, and at what dilution?
bs-8872R-Cy7 is validated for WB (1:500-2000); FCM (1:100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CYP2D6 Polyclonal Antibody, Cy7 Conjugated (bs-8872R-Cy7) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Horse, Rabbit.
How should bs-8872R-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CYP2D6 Polyclonal Antibody, Cy7 Conjugated (bs-8872R-Cy7)?
KLH conjugated synthetic peptide derived from human CYP2D6
What are the excitation and emission wavelengths of the Cy7 conjugate (bs-8872R-Cy7)?
Cy7 on bs-8872R-Cy7: excitation / emission 743nm/767nm.