CD34 Polyclonal Antibody, AbBy Fluor-647 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-8996R-BF647-100uL |
| Product Name | CD34 Polyclonal Antibody, AbBy Fluor-647 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
|
| Reactivity | Human, Mouse, Rat, Pig |
| Specifications | |
| Conjugation | AbBy Fluor-647 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CD34 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 947 |
| Swiss Prot | P28906 |
| Subcellular location | Extracellular |
| Synonyms | Hematopoietic progenitor cell antigen CD34; CD34 |
| Background | Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is CD34 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-8996R-BF647) validated for, and at what dilution?
bs-8996R-BF647 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CD34 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-8996R-BF647) react with?
Tested reactivity: Human, Mouse, Rat, Pig.
How should bs-8996R-BF647 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CD34 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-8996R-BF647)?
KLH conjugated synthetic peptide derived from human CD34
What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bs-8996R-BF647)?
AbBy Fluor-647 on bs-8996R-BF647: excitation / emission 650nm/665nm.