Annexin VI Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-8997R-Cy3 |
| Product Name | Annexin VI Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF
|
| Specificity | May have slight cross reactivity with ras-related protein Rap-2a due to 6 amino acids sequence similarity. |
| Reactivity | Human, Mouse |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Annexin VI |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 309 |
| Subcellular location | Cell membrane |
| Synonyms | Annexin VI; 67 kDa calelectrin; Annexin A6; Annexin VI p68; AnnexinA6; AnnexinVI; ANX 6; ANX A6; ANX6; ANXA 6; ANXA6; Calcium binding protein p68; Calelectrin; Calphobindin II; CalphobindinII; CBP 68; CBP68; Chromobindin 20; Chromobindin20; CPB II; CPBII; Lipocortin VI; LipocortinVI; p68; P70; Protein III; ProteinIII. |
| Background | The Annexin family of calcium binding proteins is composed of at least ten mammalian genes. It is characterised by a conserved core domain which binds to phospholipids in a Ca2+ dependent manner and a unique amino terminal region which may confer binding specificity. The Annexin family has been implicated as regulators of such diverse processes as ion flux, endocytosis and exocytosis, and cellular adhesion. When overexpressed in A431 cells, Annexin VI causes a partial reversal of the transformed phenotype. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF | IHC-P1:50-200 |
Frequently asked questions
Which applications is Annexin VI Polyclonal Antibody, Cy3 Conjugated (bs-8997R-Cy3) validated for, and at what dilution?
bs-8997R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Annexin VI Polyclonal Antibody, Cy3 Conjugated (bs-8997R-Cy3) react with?
Tested reactivity: Human, Mouse.
How should bs-8997R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Annexin VI Polyclonal Antibody, Cy3 Conjugated (bs-8997R-Cy3)?
KLH conjugated synthetic peptide derived from human Annexin VI
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-8997R-Cy3)?
Cy3 on bs-8997R-Cy3: excitation / emission 512,550nm/570,615nm.