ORC1L/ORC1 Polyclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bs-8998R-100uL |
| Product Name | ORC1L/ORC1 Polyclonal Antibody |
| Applications |
ELISA
IHC-P
IHC-F
IF
ICC/IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ORC1L/ORC1 |
| Immunogen Range | 701-800/861 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 4998 |
| Synonyms | HSORC1; MmORC1; orc1; ORC1_HUMAN; ORC1L; Origin Recognition Complex 1; Origin recognition complex subunit 1 yeast homolog like; Origin recognition complex subunit 1; Origin recognition complex subunit 1 homolog; Origin recognition complex subunit 1 like S. cerevisia; Origin recognition complex subunit 1 like; Origin recognition complex subunit 1 S. cerevisiae homolog like; Origin recognition complex, subunit 1 like yeast; PARC1; Replication control protein 1. |
| Background | The initiation of DNA replication is a multi-step process that depends on the formation of pre-replication complexes, which trigger initiation (1). Among the proteins required for establishing these complexes are the origin recognition complex (ORC) proteins (1). ORC proteins bind specifically to origins of replication where they serve as scaffold for the assembly of additional initiation factors (1). Human ORC subunits 1-6 are expressed in the nucleus of proliferating cells and tissues, such as the testis (2). ORC1 and ORC2 are both expressed at equivalent concentrations throughout the cell cycle; however, only ORC2 remains stably bound to chromatin (3,4). ORC4 and ORC6 are also expressed constantly throughout the cell cycle (5,6). ORC2, ORC3, ORC4 and ORC5 form a core complex upon which ORC6 and ORC1 assemble (7,8). The formation of this core complex suggests that ORC proteins play a crucial role in the G1-S transition in mammalian cells (8). |
| Application Dilution | |
| ELISA | WB1:300-5000 |
| IHC-P | 1:200-400 |
| IHC-F | IFIHC-P1:50-200 |
| IF | |
| ICC/IF | |
Frequently asked questions
Which applications is ORC1L/ORC1 Polyclonal Antibody (bs-8998R) validated for, and at what dilution?
bs-8998R is validated for WB (1:300-5000); IHC-P (1:200-400); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ORC1L/ORC1 Polyclonal Antibody (bs-8998R) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-8998R be stored?
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ORC1L/ORC1 Polyclonal Antibody (bs-8998R)?
KLH conjugated synthetic peptide derived from human ORC1L/ORC1 Immunogen range: 701-800/861.