ORC1L/ORC1 Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-8998R-Cy3-100uL |
| Product Name | ORC1L/ORC1 Polyclonal Antibody, Cy3 Conjugated |
| Applications |
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ORC1L/ORC1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4998 |
| Synonyms | HSORC1; MmORC1; orc1; ORC1_HUMAN; ORC1L; Origin Recognition Complex 1; Origin recognition complex subunit 1 yeast homolog like; Origin recognition complex subunit 1; Origin recognition complex subunit 1 homolog; Origin recognition complex subunit 1 like S. cerevisia; Origin recognition complex subunit 1 like; Origin recognition complex subunit 1 S. cerevisiae homolog like; Origin recognition complex, subunit 1 like yeast; PARC1; Replication control protein 1. |
| Background | The initiation of DNA replication is a multi-step process that depends on the formation of pre-replication complexes, which trigger initiation (1). Among the proteins required for establishing these complexes are the origin recognition complex (ORC) proteins (1). ORC proteins bind specifically to origins of replication where they serve as scaffold for the assembly of additional initiation factors (1). Human ORC subunits 1-6 are expressed in the nucleus of proliferating cells and tissues, such as the testis (2). ORC1 and ORC2 are both expressed at equivalent concentrations throughout the cell cycle; however, only ORC2 remains stably bound to chromatin (3,4). ORC4 and ORC6 are also expressed constantly throughout the cell cycle (5,6). ORC2, ORC3, ORC4 and ORC5 form a core complex upon which ORC6 and ORC1 assemble (7,8). The formation of this core complex suggests that ORC proteins play a crucial role in the G1-S transition in mammalian cells (8). |
| Application Dilution | |
| IF | WB1:300-5000 |
Frequently asked questions
Which applications is ORC1L/ORC1 Polyclonal Antibody, Cy3 Conjugated (bs-8998R-Cy3) validated for, and at what dilution?
bs-8998R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ORC1L/ORC1 Polyclonal Antibody, Cy3 Conjugated (bs-8998R-Cy3) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-8998R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ORC1L/ORC1 Polyclonal Antibody, Cy3 Conjugated (bs-8998R-Cy3)?
KLH conjugated synthetic peptide derived from human ORC1L/ORC1
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-8998R-Cy3)?
Cy3 on bs-8998R-Cy3: excitation / emission 512,550nm/570,615nm.