IQCJ Polyclonal Antibody

$Antigen: bs-9021P, 0.2ug\/100ul \\n\\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\n\\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\n\\nTMB staining;\\n\\nRead the data in MicroplateReader by 450$

Antigen: bs-9021P, 0.2ug\/100ul \\n\\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\n\\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\n\\nTMB staining;\\n\\nRead the data in MicroplateReader by 450

Lane 1: human colon carcinoma lysates Lane 2: mouse intestine lysates probed with Anti IQCJ Polyclonal Antibody, Unconjugated (bs-9021R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 18kD. Observed band size: 18kD.

Lane 1: Mouse Cerebrum lysates; Lane 2: Rat Cerebrum lysates probed with IQCJ Polyclonal Antibody, Unconjugated (bs-9021R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
IHC-P
IF(IHC-P)

Reactivity

Mouse
Rat

Predicted Reactivity

Human
Dog
Pig
Horse
Rabbit

Overview
Catalog #	bs-9021R
Product Name	IQCJ Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IF(IHC-P)
Reactivity	Mouse, Rat
Predicted Reactivity	Human, Dog, Pig, Horse, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human IQCJ
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	654502
Swiss Prot	Q1A5X6
Synonyms	IQ domain-containing protein J; IQCJ
Background	IQCJ (IQ domain-containing protein J) is a 159 amino acid protein that contains one IQ domain. Existing as four alternatively spliced isoforms, the gene encoding IQCJ maps to human chromosome 3q25.32. Chromosome 3 houses over 1,100 genes, including a chemokine receptor (CKR) gene cluster and a variety of human cancer-related gene loci. Key tumor suppressing genes on chromosome 3 include those that encode the apoptosis mediator RASSF1, the cell migration regulator HYAL1 and the angiogenesis suppressor SEMA3B. Marfan Syndrome, porphyria, von Hippel-Lindau syndrome, osteogenesis imperfecta and Charcot-Marie-Tooth Disease are a few of the numerous genetic diseases associated with chromosome 3.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IF(IHC-P)	1:50-200