IQCG Polyclonal Antibody, PE Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-9022R-PE-100uL |
| Product Name | IQCG Polyclonal Antibody, PE Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | PE |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human IQCG |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 84223 |
| Synonyms | IQ domain containing protein G; IQ mot containing G; DKFZp434B227; FLJ11667; FLJ23571; FLJ37775; IQCG_HUMAN. |
| Background | IQCG is a 443 amino acid protein containing one IQ domain. Widely distributed in nature, the IQ domain forms an amphiphilic seven-turn -helix capable of binding calmodulin in a Ca2+-independent manner. The level of intracellular calcium is tightly regulated in all eukaryotic cells. A modest increase in this level can result in a myriad of physiological responses, most of which are mediated by calmodulin (CaM), the universal calcium sensor. In acute T-lymphoid/myeloid leukemia, IQCG forms a complex with Nup98, an O-linked glycoprotein and a component of the nuclear pore complex. NUP98-IQCG complex bind co-activators and/or co-repressors, which suggest a role in transcriptional regulation.Nup98-IQCG complex inhibits 32Dcl3 cell apoptosis induced by Arabinofuranosylcytosine (Ara-C) and partially blocks granulocyte differentiation induced by G-CSF. IQCG exists as two isoforms due to alternatively splicing events. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | |
Frequently asked questions
Which applications is IQCG Polyclonal Antibody, PE Conjugated (bs-9022R-PE) validated for, and at what dilution?
bs-9022R-PE is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does IQCG Polyclonal Antibody, PE Conjugated (bs-9022R-PE) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Rabbit.
How should bs-9022R-PE be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise IQCG Polyclonal Antibody, PE Conjugated (bs-9022R-PE)?
KLH conjugated synthetic peptide derived from human IQCG
What are the excitation and emission wavelengths of the PE conjugate (bs-9022R-PE)?
PE on bs-9022R-PE: excitation / emission 496,564nm/578nm.