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ISLR2 Polyclonal Antibody, Cy7 Conjugated

Applications

  • WB
  • IF

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bs-9025R-Cy7
Product Name ISLR2 Polyclonal Antibody, Cy7 Conjugated
Applications
WB IF
Reactivity Human, Mouse, Rat
Specifications
Conjugation Cy7
Host Rabbit
Source KLH conjugated synthetic peptide derived from human ISLR2
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 57611
Subcellular location Extracellular
Synonyms Immunoglobulin superfamily containing leucine-rich repeat protein 2; Leucine-rich repeat domain and immunoglobulin domain-containing axon extension protein; ISLR2_HUMAN.
Background The leucine-rich (LRR) repeat is a 20-30 amino acid motif that forms a hydrophobic å/ horseshoe fold, allowing it to accommodate several leucine residues within a tightly packed core. All LRR repeats contain a variable segment and a highly conserved segment, the latter of which accounts for 11 or 12 residues of the entire LRR motif. ISLR2 (immunoglobulin superfamily containing leucine-rich repeat 2), also known as LINX (leucine-rich repeat domain and immunoglobulin domain-containing axon extension protein), is a 745 amino acid single-pass membrane protein that contains five LRR repeats, one Ig-like (immunoglobulin-like) domain, a LRRCT domain and one LRRNT domain. ISLR2 exists as a homomultimer and is essential for axon extension during neural development. The gene encoding ISLR2 maps to human chromosome 15q24.1.
Application Dilution
WB 1:300-5000
IF IHC-P1:50-200

Frequently asked questions

Which applications is ISLR2 Polyclonal Antibody, Cy7 Conjugated (bs-9025R-Cy7) validated for, and at what dilution?

bs-9025R-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does ISLR2 Polyclonal Antibody, Cy7 Conjugated (bs-9025R-Cy7) react with?

Tested reactivity: Human, Mouse, Rat.

How should bs-9025R-Cy7 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise ISLR2 Polyclonal Antibody, Cy7 Conjugated (bs-9025R-Cy7)?

KLH conjugated synthetic peptide derived from human ISLR2

What are the excitation and emission wavelengths of the Cy7 conjugate (bs-9025R-Cy7)?

Cy7 on bs-9025R-Cy7: excitation / emission 743nm/767nm.