PCID2 Polyclonal Antibody, APC-Cy7 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-9034r-apc-cy7-100uL |
| Product Name | PCID2 Polyclonal Antibody, APC-Cy7 Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | APC-Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human PCID2 |
| Immunogen Range | 101-200/399 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 55795 |
| Synonyms | CSN12 like protein; FLJ11305; PCI domain containing protein 2; PCID2_HUMAN. |
| Background | Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. May favor virus entry in case of infection with herpes simplex virus 1 (HSV1) or herpes simplex virus 2 (HSV2). |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is PCID2 Polyclonal Antibody, APC-Cy7 Conjugated (bs-9034R-APC-Cy7) validated for, and at what dilution?
bs-9034R-APC-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PCID2 Polyclonal Antibody, APC-Cy7 Conjugated (bs-9034R-APC-Cy7) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-9034R-APC-Cy7 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PCID2 Polyclonal Antibody, APC-Cy7 Conjugated (bs-9034R-APC-Cy7)?
KLH conjugated synthetic peptide derived from human PCID2 Immunogen range: 101-200/399.
What are the excitation and emission wavelengths of the APC-Cy7 conjugate (bs-9034R-APC-Cy7)?
APC-Cy7 on bs-9034R-APC-Cy7: excitation / emission 650nm/780nm.