TRPM5 Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-9047R-Cy3 |
| Product Name | TRPM5 Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat, Cow |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human TRPM5 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 29850 |
| Subcellular location | Extracellular |
| Synonyms | MLSN1 and TRP related gene 1; MLSN1 and TRP related gene 1 protein; MLSN1 and TRP-related; MLSN1- and TRP-related gene 1 protein; MTR1; Novel protein similar to vertebrate transient receptor potential cation channel, subfamily M, member 5 ; Transient receptor potential cation channel subfamily M member 5; Transient receptor potential cation channel, subfamily M, member 5; Trpm5; TRPM5 transient receptor potential cation channel, subfamily M, member 5; TRPM5_HUMAN; 9430099A16Rik; Long transient receptor potential channel 5; LTrpC-5; LTrpC5. |
| Background | Voltage-modulated Ca(2+)-activated, monovalent cation channel (VCAM) that mediates a transient membrane depolarization and plays a central role in taste transduction. Monovalent-specific, non-selective cation channel that mediates the transport of Na(+), K(+) and Cs(+) ions equally well. Activated directly by increases in intracellular Ca(2+), but is impermeable to it. Gating is voltage-dependent and displays rapid activation and deactivation kinetics upon channel stimulation even during sustained elevations in Ca(2+). Also activated by a fast intracellular Ca(2+) increase in response to inositol 1,4,5-triphosphate-producing receptor agonists. The channel is blocked by extracellular acidification. External acidification has 2 effects, a fast reversible block of the current and a slower irreversible enhancement of current inactivation. Is a highly temperature-sensitive, heat activated channel showing a steep increase of inward currents at temperatures between 15 and 35 degrees Celsius. Heat activation is due to a shift of the voltage-dependent activation curve to negative potentials. Activated by arachidonic acid in vitro. May be involved in perception of bitter, sweet and umami tastes. May also be involved in sensing semiochemicals. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is TRPM5 Polyclonal Antibody, Cy3 Conjugated (bs-9047R-Cy3) validated for, and at what dilution?
bs-9047R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does TRPM5 Polyclonal Antibody, Cy3 Conjugated (bs-9047R-Cy3) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Cow.
How should bs-9047R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise TRPM5 Polyclonal Antibody, Cy3 Conjugated (bs-9047R-Cy3)?
KLH conjugated synthetic peptide derived from human TRPM5
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-9047R-Cy3)?
Cy3 on bs-9047R-Cy3: excitation / emission 512,550nm/570,615nm.